PCR inhibitors
Ethanol re-precipitation removes PCR inhibitors from Ancient DNA extract
By: RAJEEV KUMAR PANDEY, DEEPANKAR PRATAP SINGH, GODI SUDHAKAR, VADLAMUDI RAGHAVENDRA RAO – One of the major problems in ancient DNA work is the presence of inhibitory substances, which hampers Taq polymerase activity. Therefore analysis of ancient DNA sample is very challenging. Here we describe a simple and competent ethanol re-precipitation based protocol for the…